EFFECT OF METAL NANOPARTICLES ON THE GROWTH OF NGOC LINH GINSENG (PANAX VIETNAMENSIS) LATERAL ROOTS CULTURED IN VITRO

Nguyễn Thị Nhật Linh, Hoàng Thanh Tùng, Vũ Thị Hiền, Vũ Quốc Luận, Nguyễn Phúc Huy, Nguyễn Hoàng Lộc, Dương Tấn Nhựt

DOI: http://dx.doi.org/10.26459/hueuni-jns.v126i1C.4524

Abstract


Panax vietnamensis (Ngoc Linh ginseng) plays critical roles in pharmaceutical industry because triterpenoid saponins from its roots produce medicine for improving health and treating many diseases. Metal nanoparticles reveal completely new or improved properties based on specific characteristics such as size, distribution and morphology compare to metal ion or salt; and their potential for in vitro plant cultures. Present study investigated the effects of metal nanoparticles including nZnO (0.5-2.5 mg/l), nAg (1-3 mg/l), and nCu (1-3 mg/l) supplemented in free-hormone-MS medium to in vitro Panax vietnamensis lateral root growth. Our results showed that metal nanoparticles have the positive effect on the growth of in vitro P. vietnamensis lateral roots with nAg, nCu, and nZnO. At different concentrations, in vitro P. vietnamensis lateral root growth also has various effects on the growth of lateral roots. In supplemented metal nanoparticle treatments, nCu is the most optimum for in vitro P. vietnamensis lateral root growth; the highest increase was obtained at 1.5 mg/l nCu treatment (99.3% lateral root formation and all root growth indexes are the highest). Besides, 2.5 mg/l nAg is also significantly noticed in ginseng root growth. However, the negative impact on the growth of the in vitro P. vietnamensis lateral roots showed when culture medium contained the highest concentration; such as the root growing inhibition of nCu and nAg above 2.5 mg/l. Especially, this decrease was higher with the application of nZnO0.5-2.5 mg/l (decrease the lateral root number) and 2.5 mg/l (decrease percent of lateral root formation).


Keywords


in vitro, lateral root culture, metal nanoparticle, MS medium, Panax vietnamensis

References


. Alain M, Kathryn LN, Matthew AM, Martine L, Nicolas G, Thierry J, Tatiana K (2008). Formation of metallic copper nanoparticles at the soil-root interface, Environmental Science and Technology, 42(5): 1766–1772.

. Bleecker AB, Kende H (2000). Ethylene: a gaseous signal molecule in plants, Annual Review of Cell and Developmental Biology, 16:1–18

. Bojarczuk K (2004). Effect of Toxic Metals on the Development of Poplar (Populus tremula L. × P. alba L.) cultured in vitro, Polish Journal of Environmental Studies, 13(2): 115-120.

. Cheng HM, Hsu HC, Chen SL, Wu WT, Kao CC, Lin LJ, Hsieh WF (2005). Efficient UV Photoluminescence from Monodispersed Secondary ZnO Colloidal Spheres Synthesized by Sol-Gel Method, Journal of Crystal Growth, 277(1–4): 192–199.

. Dietz KJ, Herth S (2011). Plant nanotoxicology, Trends in Plant Science, 16: 582–589.

. Do Truong Thien, Nguyen Van Tuyen, Tran Thi Y Nhi (2007). Synthesis and characterization of copper nanoparticle – chitosan, Journal of Chemistry, 45(5): 638–641.

. Duncan DB (1995). Multiple range and multiple F tests, Biometrics, 11(1): 1–42

. Dương Tấn Nhựt (2015). Biotechnology in Ngoc Linh ginseng research (Panax vietnamensis Ha et Grushv.), Ha Noi national university press.

. Hoang Thanh Tung, Nguyen Phuc Huy, Nguyen Ba Nam, Vu Quoc Luan, Truong Thi Bich Phuong, Duong Tan Nhut (2016). Effects of nano silver on growth of Chrysanthemum morifolium in microponic system, Vietnam Journal of Biotechnology, 14(3): 461–471.

. Jeong CS, Chakrabarty D, Hahn EJ, Lee HL, Paek KY (2006). Effects of oxygen, carbon dioxide and ethylene on growth and bioactive compound production in bioreactor culture of ginseng adventitious roots, Biochemical Engineering Journal, 27(3): 252–263.

. Jie Y, Weidong C, Yukui R (2017). Interactions between nanoparticles and plants: phytotoxicity and defense mechanisms, Journal of Plant Interactions, 12(1): 158–169.

. Khwaja SS, Azamal H (2017). Plant response to engineered metal oxide nanoparticles, Nanoscale Research Letters, 12(1): 92–110.

. Lin D, Xing B (2008). Root uptake and phytotoxicity of ZnO nanoparticles, Environmental Science and Technology, 42(15): 5580–5585.

. López-Moreno ML, de-la-Rosa G, Hernández-Viezcas JA, Castillo-Michel H, Botez CE, Peralta-Videa JR, Gardea-Torresdey JL (2010). Evidence of the differential biotransformation and genotoxicity of ZnO and CeO2 nanoparticles on soybean (Glycine max) plants, Environmental Science and Technology, 44(19): 7315–7320.

. Mohammad BA, Eun-Joo H, Kee-Yoeup P (2006). Copper-induced changes in the growth, oxidative metabolism, and saponin production in suspension culture roots of Panax ginseng in bioreactors, Plant Cell Reports, 25(10): 1122–1132.

. Moore R, McClelen CE (1983). A morphometric analysis of cellular differentiation in the root cap of Zea mays, American Journal of Botany, 70(4): 611–617.

. Murashige T, Skoog F (1962). A reivsed medium for rapid growth and bioassays with tobacco tissue cultures, Plant Physiology, 15(3): 473-497.

. Pokhrel LR, Dubey B (2013). Evaluation of developmental responses of two crop plants exposed to silver and zinc oxide nanoparticles, Science of the Total Environment, 452–453: 321–332.

. Raliya R, Nair R, Chavalmane S, Wang WN, Biswas P (2015). Mechanistic evaluation of translocation and physiological impact of titanium dioxide and zinc oxide nanoparticles on the tomato (Solanum lycopersicum L.) plant, Metallomics, 7(12): 1584–1594.

. Raliya R, Tarafdar JC (2013). ZnO nanoparticle biosynthesis and its effect on phosphorous mobilizing enzyme secretion and gum contents in Clusterbean (Cyamopsis tetragonoloba L.), Agriculture Research, 2(1): 48–57.

. Rani PU, Yasur J, Loke KS, Dutta D (2016). Effect of synthetic and bio synthesized silver nanoparticles on growth, physiology and oxidative stress of water hyacinth: Eichhornia crassipes (Mart) Solms, Acta Physiologiae Plantarum, 38(2): 58–61.

. Van Cuong Nguyen, Ngoc Lam Giang Nguyen, Quoc Hue Pho (2015). Preparation of magnetic composite based on zinc oxide nanoparticles and chitosan as a photocatalyst for removal of reactive blue 198, Advances in Natural Sciences: Nanoscience and Nanotechnology, 6(3): 1-8.

. Yu KW (2000). Production of the Useful Metabolites through Bioreactor Culture of Korean Ginseng (Panax ginseng C. A. Meyer), Doctor Thesis, Chungbuk National University, Korea.

. Zahed H, Ghazala M, Setsuko K (2015). Plant Responses to Nanoparticle Stress, International Journal of Molecular Sciences, 16(11): 26644–26653.